KLONOWANIE DNA PDF

Blog. 18 December Prezi Awards The best presentations have arrived. 5 December Do this, not that: Keynote speech. 28 November Wady i Zalety Klonowania Idea 1. Idea 2. Rozmnażanie bezpłciowe – naturalne klonowanie. Klonowanie zachodzi przez: Klonowanie DNA. KLONOWANIE Klony DNA służą do: Co to jest klonowanie? Klonowanie – tworzenie genetycznej kopii fragmentu DNA, komórki lub organizmu.

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We wanna make copies of this right over here.

File:Gene cloning PL.svg

A short list of examples includes:. Bacteria carrying the plasmid are selected and grown up.

Let me write the labels down, into our plasmid that also contained a gene that gave antibiotic resistance to any bacteria that takes up the plasmid. Klonowanie i rekombinacja DNA.

A common method uses two types of enzymes: And there’s a bunch of restriction enzymes, and I personally find it fascinating that we as a civilization have gotten to the point that we can find and identify these enzymes and we know at what points of DNA that they can cut. Uses of DNA cloning. The molecules extracted from the cells are applied to a column that contains antibodies specific for the target protein.

The bacteria in the large culture are induced to express the target gene through addition of a chemical signal to the culture medium. You put these plasmids in the presence of the bacteria or you provide some type of a shock, maybe a heat shock, so that some of the bacteria takes it up and then the bacteria starts reproducing. So you don’t want that one.

Klonowanie- Tworzenie genetycznych kopii by Klaudia Dula on Prezi

You have a vial and it has a solution in it with a bunch of E. For instance, when we try to insert a gene into a plasmid using a particular restriction enzyme, we may get some cases where the plasmid closes back up without taking in the geneand other cases where the gene goes in backwards.

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Bacteria contain many proteins and macromolecules. So you might have an overhang over there, you might have an overhang over there. A randomised, double-blind, placebo-controlled, phase 2b trial. But when we talk about cloning and DNA cloning we’re talking about something a little bit simpler.

See the article on restriction enzymes and DNA ligase for a more concrete example of how and why these klpnowanie ligation products can form. Most bacteria do not take up a plasmid, but some do. So how do you select for the bacteria that actually took up the plasmid?

Using a carefully chosen restriction enzyme, we digest:. Expression of the gene leads to production of mRNA, which is translated into protein. And so you won’t know, hey when this bacteria, when it keeps replicating it might form one of these, it might form one of these colonies. Now the next question, and I’m over simplifying things fairly dramatically is well you now have a bunch of bacteria that have a bunch of copies of that gene, how do you make use of it?

What’s the dnx of all that transforming, selecting, and analyzing? Cutting and pasting DNA. For instance, if our plasmid contained the human insulin gene, the bacteria would start transcribing the gene and translating the mRNA to produce many molecules of human insulin protein.

If two molecules have matching overhangs, they can base-pair and stick together. Klonowanie i rekombinacja DNA. In some cases, plasmids are directly used for practical purposes. Thus, in every ligation, we will get some number of “good” plasmids and some number of “bad” ones.

Instead, we must collect DNA from several colonies and see whether each one contain the right plasmid. A restriction enzyme is a DNA-cutting enzyme that recognizes a specific target sequence and cuts DNA into two pieces at or near that site. Each surviving bacterium will give rise to a small, dot-like group, or colonyof identical bacteria that all carry the same plasmid.

During transformationspecially prepared bacterial cells are given a shock such as high temperature that encourages them to take up foreign DNA. Once the protein has been produced, the bacterial cells can be split open to release it. DNA molecules built through cloning techniques are used for many purposes in molecular biology.

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It’s identical copies of a piece of DNA. They’re not even going to happen.

Because of this, the newly made protein needs to be purified separated from the other proteins and macromolecules before it can be used. Thus, bacteria that took up the plasmid can be selected on nutrient plates containing the antibiotic. A plasmid typically contains an antibiotic resistance genewhich allows bacteria to survive in the presence of a specific antibiotic.

Well, the bacteria themselves, let’s koonowanie that gene is for something you want to manufacture say insulin for diabetics, well you could actually use that bacteria’s machinery, we used its reproductive machinery to keep replicating the genetic information, but you can also use its productive machinery, I guess you could say, it’s going to express its existing DNA but it can also express the genes that are on the plasmid.

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DNA cloning can be used to make human proteins with biomedical applications, such as the insulin mentioned above. The plasmid is introduced into bacteria via process called transformationand bacteria carrying the plasmid are selected using antibiotics.

Colonies with the right plasmid can be grown to make large cultures of identical bacteria, which klonowaniw used to produce plasmid or make protein. So now you have a gene for antibiotic resistance here, and so only the bacteria, and I think it’s amazing that we as humanity are able to do these types of things, but now only the bacteria that have taken up the plasmid will have that antibiotic resistance.

The restriction enzymes are just in mass cutting these things.