Disease. Gummy stem blight. Causes leaf spot, stem canker, black leaf and fruit rot; common name refers to the characteristic gummy exudate. Didymella bryoniae (Auersw.) Rehm [anamorph Phoma cucurbitacearum (Fr.) Sacc.] is the causative agent of gummy stem blight (GSB), a disease affecting. Gummy stem blight of cucurbits produces a variety of symptoms which are referred to as leaf spot, stem canker, vine wilt and black fruit rot. Lesions on leaves.
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Calcein was bought from Sigma-Aldrich Co. Rehm didymellz Phoma cucurbitacearum Fr.: Rapid and precise detection of latent infections of wheat stripe rust in wheat leaves using loop-mediated isothermal amplification. Hence, this study represents a successful attempt to develop a LAMP-based detection method of infection in early cucurbit crops. On most hosts, more than half of the spots start at the margins of leaves or extend to the margins Fig.
From native plants in central Europe to cultivated crops worldwide The emergence of Didymella bryoniae as a cucurbit pathogen. An improved real-time PCR system for broad-spectrum detection of Didymella bryoniaethe causal agent of gummy stem blight of cucurbits.
This improved the reaction time and efficiency. Role of insect injury and powdery mildew in the epidemiology of the gummy stem blight disease of cucurbits. Lanes 1 and 2, purified DNA from D.
Plant Health Progress doi: In A,Blane 1: Identification and characterization of the causal agent of gummy stem blight from muskmelon and watermelon in East China. A photoperiod of 16 h of light is required to induce rapid production of fruiting bodies 10, LAMP products can easily be visualized by gel electrophoresis or by measuring turbidity didyella by a white precipitate of magnesium pyrophosphate Notomi et al.
Benincasa hispida wax gourd. Therefore, this assay could be useful even for amateur farmers without the need for elaborate laboratory equipment.
The sensitivity was consistent with a previous report for LAMP methods used to detect Sclerotinia sclerotiorum Duan et al. Morphological, pathological, and genetic differentiation of Didymella bryoniae and Phoma spp. The potential application of this diagnostic tool will enable early prediction of disease, reducing the risks of epidemics.
As of 1 Januarychanges to the International Code of Botanical Nomenclature specify that each fungus is to be known by only one name, even if it has teleomorphic sexually reproducing and anamorphic asexual states. Published 24 October The most common methods currently used for the rapid detection of D.
To didtmella this, visualization of indirect colorimetric indicators such as hydroxynaphthol blue or calcein have been used as an improvement Niessen and Vogel, ; Duan et al. Supplementary Material The Supplementary Material for this article can be found online at: Rankings of hosts also differ depending on whether susceptibility to foliar blight, crown, stem and vine cankers, or fruit rot is considered. Didymella bryoniae Symptoms of D.
The anamorph recently was renamed Stagonosporopsis cucurbitacearum Fr. Gummy exudates may occur from cracks, especially in watermelon and pumpkin. Although this coloration is not specific to D.
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Compared with reported LAMP assays and conventional PCR, the LAMP assay reported here is more advantageous owing to its sensitivity and efficiency, and is robust enough to be used in latently infected crop testing applications in the field.
Didymella bryoniae is a pathogenic fungus that causes gummy stem blight GSB in Cucurbitaceae crops e. Conflict of Interest Statement The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
All parts byoniae the plant can display symptoms and signs but the roots.
Morphological, pathological, and genetic differentiation of Bryonuae bryoniae and Phoma spp. Cultures on filter paper will remain viable up to 10 years. Using the optimal reaction conditions described above, and based on the presence of a PCR product of the expected size, the D. A set of three conventional PCR primers is available to identify D.